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Substrate Recipes for Maximizing Mushroom Yields

A detailed visual guide on how to make mushroom substrate using grain spawn, pasteurised straw, compost, and hardwood sawdust.

Innovative Substrate Recipes for Maximizing Mushroom Yields

Mushroom cultivation is both an art and a science, one of the most critical factors influencing success is the substrate—the material that serves as the growing medium for mushrooms. Substrates provide the nutrients mushrooms need to thrive, and experimenting with innovative substrate recipes can significantly boost yields. This guide explores cutting-edge ideas for maximizing mushroom yields and includes a hands-on experiment for creating a high-yield mushroom substrate at home in the UK.

What Makes a Good Mushroom Substrate?

A great mushroom substrate balances the following factors:
  1. Nutritional Content: Mushrooms require carbohydrates, nitrogen, and minerals for optimal growth. Materials like straw, sawdust, and Organic rye grain provide these essential nutrients.
  2. Moisture Retention: Mushrooms thrive in high-humidity environments. A substrate should retain enough moisture without becoming waterlogged.
  3. Aeration: Proper airflow prevents anaerobic conditions, which can lead to contamination. Materials like vermiculite or perlite improve substrate texture and aeration.
  4. Contamination Resistance: Substrates should either resist contamination like Coco coir or be easy to sterilize like Straw. Pasteurization or sterilization is essential for ensuring a clean growing environment.

Innovative Substrate Recipes for Maximizing Mushroom Yields

1. Enriched Straw Substrate.

  • Oyster Mushrooms (Pleurotus ostreatus)
  • King Oyster Mushrooms (Pleurotus eryngii)
  • Golden Oyster Mushrooms (Pleurotus citrinopileatus)
  • Pink Oyster Mushrooms (Pleurotus djamor)
  • Wine Cap Mushrooms (Stropharia rugosoannulata)
These strains thrive on enriched straw substrates due to their preference for nutrient-dense, fibrous growing mediums. Adding nitrogen-rich additives like coffee grounds or alfalfa meal further boosts their growth and yield.
To prepare mushroom substrate for Oyster mushrooms:
    • 2 parts chopped wheat or barley straw
    • 1 part coffee grounds (sterilized)
    • 1 tablespoon gypsum per 5 kilograms of substrate

2. Hardwood Sawdust Blend

  • Shiitake Mushrooms (Lentinula edodes)
  • Lion’s Mane Mushrooms (Hericium erinaceus)
  • Reishi Mushrooms (Ganoderma lucidum)
  • Maitake Mushrooms (Grifola frondosa)
  • Chestnut Mushrooms (Pholiota adiposa)
Hardwood sawdust is ideal for species like shiitake and lion’s mane. Adding bran or soybean hulls boosts its nutritional profile, supporting larger flushes.
  • To prepare mushroom substrate for Lions Mane:
    • 4 parts hardwood sawdust (oak or beech)
    • 1 part wheat bran or soybean hulls
    • 2% gypsum (by weight)

3. Coco Coir and Vermiculite Mix

  • Psilocybe cubensis
  • Golden Teacher Mushrooms (Psilocybe cubensis variant)
  • B+ Mushrooms (Psilocybe cubensis variant)
  • Blue Meanies (Psilocybe cubensis variant)
  • Albino A+ Mushrooms (Psilocybe cubensis variant)
This substrate is popular for its versatility and ease of use. It works well for species like Psilocybe cubensis.
  • To prepare mushroom substrate commonly used in Monotub growing:
    • 1 brick of coco coir (hydrated)
    • 2 parts vermiculite
    • 5% gypsum (optional)

4. Spent Brewer’s Grain

  • Oyster Mushrooms (Pleurotus ostreatus)
  • Lion’s Mane Mushrooms (Hericium erinaceus)
  • Wine Cap Mushrooms (Stropharia rugosoannulata)
  • King Oyster Mushrooms (Pleurotus eryngii)
  • Shiitake Mushrooms (Lentinula edodes)
Recycling spent grains from breweries is an eco-friendly and nutrient-dense option for mushroom growers.
  • Recipe:
    • 2 parts spent brewer’s grain (sterilized)
    • 1 part straw or coir
    • 1 tablespoon gypsum per 5 kilograms of substrate

5. Manure-Based Substrates

  • Button Mushrooms (Agaricus bisporus)
  • Portobello Mushrooms (Agaricus bisporus, mature stage)
  • Crimini Mushrooms (Agaricus bisporus, intermediate stage)
  • Psilocybe cubensis
  • Stropharia Mushrooms (Stropharia rugosoannulata)
These strains excel on manure-based substrates due to their ability to utilize the rich nutrients and organic matter found in aged and composted manure, supporting vigorous growth and high yields.
  • Recipe:
    • 2 parts aged horse or cow manure
    • 1 part straw
    • 1 part coco coir
Fancy skipping all of the work and want to try one of our Inject and Forget Auto Mushroom growing bags?

Follow-Along Experiment: High-Yield Mushroom Substrate at Home.

  • Psilocybe cubensis
  • Golden Teacher Mushrooms (Psilocybe cubensis variant)
  • B+ Mushrooms (Psilocybe cubensis variant)
  • Albino A+ Mushrooms (Psilocybe cubensis variant)
  • Blue Meanies (Psilocybe cubensis variant)

Objective:

Create a high-yield mushroom substrate using materials readily available in the UK.

Materials Needed:

Steps:

1. Prepare Mushroom Substrate using Coco Coir:
  1. Place the Coco coir brick into the large bucket.
  2. Pour 4 liters of boiling water over the brick and cover the bucket with a lid.
  3. Let it sit for 30 minutes, allowing the coir to fully hydrate.
  4. Fluff the coir with a clean spatula or gloved hands.
2. Mix the Substrate:
  1. Add the vermiculite, coffee grounds, and gypsum to the hydrated coir.
  2. Mix thoroughly until all components are evenly distributed.
3. Test for Field capacity aka Moisture Content:
  1. Take a handful of the substrate and squeeze it. If a few drops of water come out, the moisture level is perfect.
  2. If water streams out, add more vermiculite to balance the moisture. If it feels too dry, sprinkle in a little more water.
4. Sterilize the Substrate:
  1. Place the substrate in resealable bags or jars.
  2. Sterilize the substrate by steaming it for 90 minutes or using a pressure cooker for 90 minutes at 15 PSI.
  3. Let the substrate cool completely before inoculating.
5. Inoculate the Substrate:
  1. Using a sterile spore syringe, inject the substrate with your chosen mushroom spores.
  2. Seal the bags or jars and incubate them in a warm, dark environment (20-27°C) until fully colonized.
6. Fruiting:
  1. Transfer the colonized substrate bags to a fruiting chamber like the GrowBuddie60 with high humidity and indirect light.
  2. Monitor daily, misting as needed, and wait for your first flush of mushrooms to appear.
You should try our Pre-mixed and Pre-sterilised Coco coir, Vermiculite and Gypsum substrate commonly known as CVG BAGS

Tips for Success

  • Sterility is Key: Contamination can ruin a batch, so always work in a clean environment and sterilize tools and materials.
  • Track Your Progress: Keep a cultivation journal to note substrate recipes, environmental conditions, and yields.
  • Experiment with Additives: Try incorporating different additives like soybean hulls or biochar to see how they affect yields.

Why Use Substrate Recipes for Maximizing Mushroom Yields?

Using innovative substrates tailored to specific mushroom species can:
  1. Maximize Yields: Providing optimal nutrients ensures larger flushes and better overall performance.
  2. Improve Efficiency: High-quality substrates support faster colonization and fruiting.
  3. Reduce Waste: Recycling materials like coffee grounds or brewer’s grain turns waste into a valuable resource.
  4. Enhance Sustainability: Eco-friendly substrates minimize environmental impact while supporting mushroom growth.

Words from the Author.

Creating high-yield mushroom substrates doesn’t require a professional lab or expensive equipment. With a little experimentation and creativity, you can optimize your growing medium to achieve impressive results at home. Whether you’re a beginner or an experienced cultivator, innovative substrates offer endless opportunities to refine your craft and boost your harvests.Try the experiment outlined above, and don’t be afraid to adapt it to suit your needs. By combining science and a touch of ingenuity, you’ll unlock the full potential of mushroom cultivation in your home. Happy growing!
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