Description
Introducing our Non-Sterile Empty Glass Jars 300ml – ideal for agar studies or making your own liquid cultures. These durable jars are the perfect blank canvas for your mycology experiments, allowing you to customize them based on your specific needs. Use them for preparing nutrient-rich agar or creating liquid cultures for faster mycelium growth. Simply add your preferred medium, sterilize in your setup, and get started with incubation. These jars come with secure, screw-on lids and can be used repeatedly for various mycological applications.
Key Features:
- Non-Sterile Empty Glass Jars: Ready to be filled with agar or liquid culture solutions.
- Durable and Reusable: High-quality jars perfect for long-term use in lab settings.
- Customizable: Add your own nutrients and sterilize as needed for your research.
- Perfect for Liquid Culture or Agar Studies: A versatile tool for growing and isolating mycelium.
How to Use for Agar or Liquid Culture:
- Prepare Agar or Nutrient Solution: Mix your nutrient solution for agar or liquid culture and pour into the jar.
- Sterilize: Place the jar in your pressure cooker or autoclave to ensure sterility.
- Inoculate: Add an agar wedge or spores after sterilization in a controlled environment like a flow hood.
- Incubate: Place at 25-26°C and shake the jar daily if using for liquid culture.
Experiment: How to Make Liquid Culture and Sterilize the Jar
Objective: Create a sterile liquid culture using a non-sterile 300ml jar, agar wedge, and nutrient solution.
Materials Needed:
- Non-Sterile 300ml Jar
- Nutrient Solution (e.g., malt extract or honey water)
- Agar Wedge with Mycelium
- Pressure Cooker or Autoclave
- Alcohol Swabs
- 14-16g Sterile Needle and Syringe
- Flame Source for Sterilization
Steps to Sterilize the Non-Sterile Empty Glass Jars:
- Prepare Nutrient Solution
- Mix your nutrient with Distilled water to make nutrient solution (such as 4% malt extract solution) and pour it into the jar, filling about 50-75% of the jar.
- Seal the Jar
- Screw the lid on but leave it slightly loose to allow pressure to escape.
- Sterilize the Jar
- Place the jar into a pressure cooker or autoclave.
- Sterilize at 15 PSI (121°C) for 20-30 minutes.
- Cool the Jar
- Allow the jar to cool down inside the pressure cooker to prevent contamination.
Steps to Make Liquid Culture:
- Inoculate the Jar
- In a sterile environment (like a still-air box or flow hood), unscrew the jar lid.
- Drop a small agar wedge with mycelium into the jar.
- Secure the Jar
- Tighten the lid. If the jar has an injection port, you can apply micro-pore tape for added security.
- Incubate
- Incubate the jar at 25-26°C for optimal growth.
- Shake the jar once daily to distribute the mycelium evenly through the nutrient solution.
- Monitor Growth
- After a few days, the mycelium should visibly colonize the liquid culture. Once 50% or more is colonized, it’s ready for use.
- Test and Use
- Test a small amount on an agar plate to ensure sterility. If clean, use a sterile needle and syringe to extract the liquid culture for inoculating bags or jars.
This method provides an efficient way to multiply mycelium for fast colonization and cultivation.
These jars are perfect for anyone looking to advance their mycology research with a versatile, customizable solution. Get yours today to start your next experiment!
