Description
Enhance Your Mushroom Cultivation with Self-Healing Injection Ports
Sold in packs of 10.
What Are Butyl rubber Injection Ports
Self healing Injection Ports are essential for mycology, ensuring a sterile environment when introducing liquid cultures or spores into a substrate. These ports are designed to automatically reseal after injection, preventing contamination in your mushroom cultivation process.
How They Work
These ports are highly effective when paired with luer lock caps for syringes, allowing for easy, sterile inoculation of spore or liquid culture syringes. The injection process is seamless, as the port reseals instantly, ensuring the content remains uncontaminated.
Why Choose Injection Ports over Chewing gum?
Injection ports provide sterile access points for your cultivation bags or jars, significantly reducing contamination risks. Paired with sterile syringe caps for mycology, they create a perfect environment for liquid culture or spore injection.
Ideal for Mycology Enthusiasts and Professionals
Whether you are a hobbyist or a professional, these self-healing mushroom culture ports simplify the inoculation process. They work well with sterile syringe end caps to ensure the highest level of protection for your growing substrate.
Key Features of Butyl rubber Injection Ports
- Sterile and contamination-free access for spore or liquid culture injection.
- Perfect for use with spore syringes.
- Designed for mushroom cultivators seeking efficient and sterile tools.
Reliable and Sterile Protection
These ports are not only ideal for injecting spores into Culture Jar Lids but also function well with MycoBox Filter lids, making them versatile for different stages of your cultivation journey.
Experiment: Creating a Liquid Culture Jar Using Self-Healing Injection Ports
Materials:
- Sterilized liquid culture jar with nutrient solution
- Self-Healing Injection Port (affixed to jar lid)
- Sterile syringe with Luer Lock End Cap
- Mushroom spore syringe or agar wedge
- Tinfoil
- Pressure cooker or autoclave
- Micropore tape
- Sterile gloves
Procedure:
1. Prepare the Liquid Culture Jar:
- Cover the top half of the jar with tinfoil so as to cover the lid completely. Sterilize the jar containing the nutrient solution with the Self-Healing Injection Port using a pressure cooker at 15 PSI for 30 minutes. Ensure the injection port is securely fixed to the jar lid before Heat Sterilization.
2. Sterilization and Setup:
- Working in a sterile space (Still air box or Flow hood)
- Wait till the jar has cooled then remove the tinfoil covering the lid.
- Prepare your spore syringe or an agar wedge.
3. Inoculate the Liquid Culture:
- If using a spore syringe: Insert the syringe needle through the Self-Healing Injection Port and inject 1-2 mL of spore solution into the nutrient solution.
- If using an agar wedge: Use a sterile tool to transfer a small agar wedge into the jar. You must be inside a sterile environment (like a Still-air box or Flow Hood ).
4. Seal and Incubate:
- Once inoculated, remove the needle. The Injection Port will automatically seal itself.
- Place a piece of micropore tape over the injection port to further secure it.
- Incubate the jar at 25-27°C for 7-14 days, shaking it once daily to promote even mycelium growth in the liquid culture.
5. Harvesting Liquid Culture:
- Once the jar is 50-80% colonized, the liquid culture is ready for use. Move into the sterile space and peel off the micropore tape to reveal a Sterile injection port. There is no need to Sterilize the injection port again as the micropore tape will keep the port clean and when removed will leave a clean surface. You can now extract the culture with a sterile syringe for further inoculation of grain spawn or substrate jars.
6. Testing the Liquid Culture:
- For safety, always test the liquid culture by inoculating a small amount onto an agar dish to ensure it is contamination-free before mass inoculation.
By using Self-Healing Injection Ports, this experiment demonstrates an efficient way to prepare liquid cultures in a contamination-free environment, ensuring faster mycelium colonization for your mushroom cultivation projects.
Order Today for a More Efficient Growing Process
Optimize your cultivation process by using self-healing injection ports. Ensure sterility, save time, and protect your cultures.
