Using a spore syringe for Microscopy.

Learn how to properly use a Golden teacher spore syringe for microscopic research. Whether you’re new to the field or fine-tuning your technique, this guide will walk you through every step to ensure safe, sterile, and effective observation of mushroom spores.

What You’ll Need

Sterile spore syringe (e.g., Golden Teacher Spore Syringe)

Clean glass microscope slides

Sterile scalpel or tweezers

Cover slips

Gloves and isopropyl alcohol

Laminar flow hood or still air box (recommended)

Microscope (1000x recommended for spore detail)

Step-by-Step Instructions

Sterilize Your Work Area
Use a 70% alcohol solution to disinfect your workspace and tools.

Wear Gloves
Avoid introducing contaminants by always wearing sterile gloves.

Attach the Needle to the Spore Syringe
Twist the sterile needle onto the syringe body. Do not touch the needle tip.

Dispense a Small Drop
Release 1–2 drops of spore solution onto a clean slide. Less is more—over-saturation can reduce clarity.

Apply a Cover Slip
Carefully place a cover slip over the spore droplet to prevent evaporation and allow even distribution.

Begin Observation
Start with low magnification (100x) to locate the spores, then switch to 400x–1000x for detailed study.

Storage Tip

After use, wipe the needle with alcohol, recap the syringe, and store it in the fridge (2–8°C) to preserve it for future research.

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